Application

Anti-LC3C antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

MAP1LC3 is essential for the formation of autophagosomes. LC3C is used as an autophagy and mitophagy marker.

Macroautophagy, usually referred to as an autophagy, is a major pathway for bulk degradation of cytoplasmic constituents and organelles. In this process, portions of the cytoplasm are sequestered into double membrane vesicles, called autophagosomes, and subsequently delivered to the lysosome for degradation and recycling. Although autophagy is a constitutive cellular event, it is enhanced under certain conditions such as starvation, hormonal stimulation and drug treatments. Autophagy plays an essential role in cellular differentiation, cell death and aging and is required for normal turnover of cellular components during starvation. Defective autophagy process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections.Autophagy pathway is evolutionary conserved in all eukaryotic cells.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

At least 16 genes encoding for autopahgy (ATG) related proteins that are required for autophagosome formation were identified in yeast by genetic screens. For many of these genes, related homologs have been identified in mammals.The Microtubule-associated protein light chain 3 (MAP1LC3), the mammalian homolog of yeast Atg8/Apg8/Aut7, is essential for the formation of autophagosomes. In addition, three human orthologs of yeast Atg8, named LC3A (MAP1LC3A), LC3B (MAP1LC3B) and LC3C (MAP1LC3C), were identified. These three human isoforms show different expression patterns in human tissues.The LC3 protein exists in two forms, cytoplasmic LC3-I (18 kDa) and autophagosome membrane associated LC3-II (16 kDa). LC3-II is the result of LC3-I cleavage at the C-terminal Glycine by cysteine protease Atg4, which is further modified by the mammalian E1- and E2-like enzymes: Atg7 and Atg3. The amount of LC3-II correlates with the extent of autophagosome formation. Thus, LC3C can be used as an autophagosomal and mitophagy marker.

Microtubule-associated protein light chain 3 (MAP1LC3) is the mammalian homolog of yeast Atg8/Apg8/Aut7. Three human orthologs of yeast Atg8, named LC3A (MAP1LC3A), LC3B (MAP1LC3B) and LC3C (MAP1LC3C), were identified. These three human isoforms show different expression patterns in human tissues. The LC3 protein exists in two forms, cytoplasmic LC3-I (18 kDa) and autophagosome membrane associated LC3-II (16 kDa).

Immunogen

Synthetic peptide corresponding to human N-terminal region LC3C, conjugated to KLH

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Specificity

Anti-LC3C antibody specifically recognizes human LC3C-I and/or LC3C-II and does not cross react with human LC3A or LC3B. 

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.